搜索熱:郭偉燦 3D打印
掃一掃 加微信
首頁 > 期刊論文 > 論文摘要
高效液相色譜法檢測水產品中磺胺類和乙胺嘧啶藥物殘留
          
HPLC Determination of Residual Sulfanilamides and Ethylaminopyrimidines in Aquatic Products

摘    要
用高效液相色譜法檢測水產品中磺胺類、乙胺嘧啶等合成抗菌劑殘留.樣品以乙腈、二氯甲烷、乙酸乙酯以體積比2比1比1為提取劑,經脫脂、凈化、濃縮,用流動相溶解.用高效液相色譜法紫外檢測器測定.通過梯度洗脫將7種磺胺類、乙胺嘧啶進行分離.各標準曲線線性范圍為0.05~2.0 mg·L-1,相關系數為0.983 7~0.997 5,回收率分別為62.2%~88.6%,相對標準偏差為5.85%~8.12%(n=7),檢出限(S/N=3)為10~50 μg·kg-1.
標    簽 高效液相色譜   磺胺類   乙胺嘧啶   High performance liquid chromatography   Sulfanilamides   Ethylaminopyrimidines  
 
Abstract
HPLC was applied to the determination of residual sulfanilamides and ethylaminopyrimidines in aquatic products.The residual drugs in the sample were extracted with a solvent mixture of acetonitrile,dichloromethan and ethyl acetate mixed in the proportion of 2∶1∶1 respectively.The extract obtained was defatted and purified by extraction with hexane,and the hexane extract was then concentrated by evaporation.The residue was taken up with a mixed solvent of acetonitrile,acetic acid(2+98) and H2O mixed in the ratio of 15+25+60,which was used also as the mobile phase in the chromatographic separation later.Seven kinds of sulfanilamide and ethylaminopyrimidine drugs were well separated by gradient elution on the chromatographic column of Clovisl-C18(4.6 mm×150 mm,5 μm),and their contents determined by UV-detection at the wavelength of 275 nm.Linear relationship between the peak area and the concentration of standards of the 7 different drugs were found all in range of 0.05-2.0 mg·L-1,with correlation coefficients in the range of 0.983 7 to 0.997 5.In the tests for precision,accuracy and sensitivity of the proposed method,the results obtained were as follows: RSD′s 5.85%-8.12%,recovery 62.2%-88.6%,detection limit(S/N=3) 10-50 μg·kg-1.

中圖分類號 O657.7

 
  中國光學期刊網論文下載說明


所屬欄目 工作簡報

基金項目 浙江省分析測試基金資助項目的子課題

收稿日期 2006/12/29

修改稿日期

網絡出版日期

作者單位點擊查看

備注李佐卿(1947-),男,寧波人,研究員,主要研究方向為色譜分析和食品理化檢驗.

引用該論文: LI Zuo-qing,NI Mei-lin,ZHANG Zai-ting,XIE Dong-hua,YU Xue-jun,ZHAN Jia,YIN Ju-yi. HPLC Determination of Residual Sulfanilamides and Ethylaminopyrimidines in Aquatic Products[J]. Physical Testing and Chemical Analysis part B:Chemical Analysis, 2008, 44(6): 557~559
李佐卿,倪梅林,章再婷,謝東華,俞雪鈞,湛嘉,殷居易. 高效液相色譜法檢測水產品中磺胺類和乙胺嘧啶藥物殘留[J]. 理化檢驗-化學分冊, 2008, 44(6): 557~559


被引情況:


【1】鄭春巍,劉洋,王啟輝,周曉萍, "超高效液相色譜法測定畜禽肉中10種磺胺類藥物殘留量",理化檢驗-化學分冊 48, 79-81(2012)



論文評價
共有人對該論文發表了看法,其中:
人認為該論文很差
人認為該論文較差
人認為該論文一般
人認為該論文較好
人認為該論文很好
分享論文
分享到新浪微博 分享到騰訊微博 分享到人人網 分享到 Google Reader 分享到百度搜藏分享到Twitter

參考文獻
【1】Sherri B T,Austin R L(美).動物源性食品中藥物殘留分析方法[M].福建省進口食品行業協會譯.天津:天津科技翻譯出版公司,1999:404.
 
【2】Spinks C A,Wyatt G M,Everest S,et al.Atypical antibody specificity: advancing the development of a generic assay for sulphonamides using heterologous ELISA[J].Journal of the Science of Food and Agriculture: 2002,82(4):428-434.
 
【3】Brown J.Compound Evaluation and Analytical Capability,National Residue Program Plan(Science and Technology Program)[M].Washington D C: USDA,Food Safety and Inspection Service,1991.
 
【4】Smedley M D,Weber J D.Liquid chromatographic determination of multiple sulfonamide residues in bovine milk[J].J Assoc Off Anal Chem,1990,73(6):875-879.
 
【5】Tsai C E,Kondo F,Ueyama Y,et al.Determination of sulfamethazine residue in chicken serum and egg by high-performance liquid chromatography with chemiluminescence detection[J].J Chromatogr Sci,1995;33(7):365-369.(上接第556頁)
 
【6】彭愛紅,覃連娜.酪氨酸、脯氨酸和組氨酸的示波極譜連續測定[J].分析試驗室,2001,20(4):25-27.
 
【7】何瓊.聚乙烯吡咯烷酮修飾碳糊電極上酪氨酸的電化學研究及其測定[J].分析科學學報,2003,19(4):346-348.
 
【8】孫延一.酪氨酸基于表面活性效應在碳糊電極上的伏安行為研究[J].理化檢驗-化學分冊,2005,41(4):229-231.
 
【9】Song Y Z.Theoretical study on the electrochemical behavior of norepinephrine at Nafion multi-walled carbon nanotubes modified pyrolytic graphite electrode[J].Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy,2007,67(5):1169-1177.
 
【10】Zhao G C,Yin Z Z,Zhang L,et al.Direct electrochemistry of cytochrome c on a multi-walled carbon nanotubes modified electrode and its electrocatalytic activity for the reduction of H2O2[J].Electrochemistry Communications,2005,7(3):256-260.
 
【11】Shahrokhian S,Reza H,Mehrjardi Z.Application of thionine-nafion supported on multi-walled carbon nanotube for preparation of a modified electrode in simultaneous Voltametric detection of dopamine and ascorbic acid[J].Electrochemical Acta,2007,52(22):6310-6317.
 
【12】Tsang S C,Chen Y K,Harris P J E,et al.A simple chemical method of opening and filling carbon nanotubes[J].Nature,1994,372(6):159-162.
 
【13】Wu K B,Wang H,Chen F,et al.Electrochemistry and voltammetry of procaine using a carbon nanotube film coated electrode[J].Bioelectrochemistry,2006,68(2):144-149.
 
相關信息
   標題 相關頻次
 高效液相色譜-串聯質譜法測定水產品中硝基呋喃代謝物
 14
 高效液相色譜-質譜聯用法檢測烤鰻中環丙沙星
 8
 動物源性食品中小分子有機污染物的多類別多殘留分析技術的研究進展
 4
 氣相色譜法測定毛豆中一些殘留殺蟲劑
 4
 氣相色譜-質譜法測定水產品中硫丹和毒死蜱的殘留量
 3
 高效液相色譜-串聯質譜法測定香腸中硝基呋喃代謝物
 2
 高效液相色譜電化學發光法檢測抗壞血酸
 2
 高效液相色譜法測定環境水中7種陰離子
 2
 高效液相色譜法測定桔梗中皂苷類物質
 2
 高效液相色譜法測定蘋果與山楂制品中展青霉素
 2
 高效液相色譜法快速測定三白草中黃酮類物質
 2
 天然酚、有機酸的高效液相色譜分析的進展
 2
 鎢合金殼體的超聲檢測及缺陷分析
 2
 鎢鉬合金的超聲檢測及其缺陷分析
 2
 液相色譜-串聯質譜法測定葡萄酒中7種生物胺含量
 2
 HPLC可作為傳統中藥學指紋分析方法 為中藥質量控制做好準備
 1
 HPLC柱壓突然升高的原因及排除
 1
 HPLC組分的快速凍干法
 1
 QuEChERS提取-高效液相色譜法測定泰山蟲草中4種黃曲霉毒素的含量
 1
 超純水水質對于LC-MS的影響
 1
 超聲提取-高效液相色譜法測定粗榧中高三尖杉酯堿
 1
 島津應用:LC-MS/MS法同時測定中藥材中153種農藥殘留
 1
 高速勻漿提取-高效液相色譜法測定煙草樣品中多酚
 1
 高效凝膠滲透色譜及高效液相色譜和電噴霧-離子阱質譜法聯用測定構成蘆薈多糖的單糖
 1
 高效液相色譜-電感耦合等離子體質譜法測定淡水中7種元素形態的含量
 1
 高效液相色譜-電感耦合等離子體質譜法測定飲用水中碘酸根和碘離子
 1
 高效液相色譜電化學檢測法測定阿奇霉素及相關組分
 1
 高效液相色譜法測定苯并三氮唑
 1
 高效液相色譜法測定動物性食品中合成色素
 1
 高效液相色譜法測定動物源食品中磺胺類藥物殘留量
 1